Journal: PLoS Pathogens

Article Title: The RNA Binding Specificity of Human APOBEC3 Proteins Resembles That of HIV-1 Nucleocapsid

doi: 10.1371/journal.ppat.1005833

Figure Lengend Snippet: (A) A3-RNA cross-linked complexes were immunoprecipitated from mock infected or HIV-1 NL4-3 ΔVif infected HEK 293T cells stably expressing 3×HA-tagged A3F, A3G or A3H proteins that had been fed with 4SU and UV-irradiated. A3-RNA cross-linked complexes were also immunoprecipitated from purified HIV-1 NL4-3 ΔVif virions that were derived from the 4SU-fed infected cells. Complexes were visualized by autoradiography (top) and Western blot analysis using a polyclonal anti-HA (bottom). (B) Frequency distribution of nucleotide occurrence (read density) in reads that were mapped to the HIV-1 NL4-3 genome (left). A colinear schematic diagram of the HIV-1 genome is presented above. Correlation analyses (see ) of A3 binding to viral RNA in cells from two independent CLIP experiments are shown (right).

Article Snippet: The rabbit polyclonal anti-HA (600-401-384 Rockland) and the mouse monoclonal anti-HIV-1 p24CA (183-H12-5C, NIH AIDS Reagent Program) were used in Western blot analyses.

Techniques: Immunoprecipitation, Infection, Stable Transfection, Expressing, Irradiation, Purification, Derivative Assay, Autoradiography, Western Blot, Binding Assay